Workshop 1: Practical enzyme kinetics: biochemical characterization of proteolysis
Guy Salvesen (Sanford-Burnham Medical Research Institute), Christian Sommerhoff (University of Munich), Scott Snipas (Sanford Burnham Prebys Medical Discovery Institute), Fernanda dos Reis Rocho (USP)
In this workshop, trainees will discuss and apply principles of theoretical and practical enzyme kinetics with a special focus on proteases. Topics include substrate and inhibitor kinetics, steady-state, pre-steady-state and transient approaches, inhibitor mechanisms, slow versus fast binding, hardware, and software. The workshop includes both seminars and wet lab experiments.
Workshop 2: Important Tools and Techniques for Protease Researchers
Anthony D’Donoghue (UCSD), Antoine Dufour (University of Calgary), Galia Blum (The Hebrew University), Olivier Julien (University of Alberta)
Protease substrate profiling, mass spectrometry-based methods, use of reporter substates, development of diagnostic assays, design of highly specific activity -probes, use of proteases to releases anti-body drug conjugates, broad spectrum ABPs, imaging active proteases in cells and tissues, theranostic applications (FAP probes), use of websites TopFIND and TopFINDER, design IceLogo, validation of protease substrates using ATOMS
Workshop 3: System-wide analysis of proteolytic events with MS-based proteomics
Konstantinos Kalogeropoulos (Technical University of Denmark), Peter Bell (University of British Columbia)
Mass spectrometry-based methods, degradomics, substrate discovery (data analysis), systems-biology, TAILs, CLIPPER
Coordination:
Ana Paula C. A. Lima and Maria Luiza Oliva